RosetteSep, Human, Progenitor Cell Pre-Enrichment Kit, Human Progenitor Cell, Human Progenitor Cell kit
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Catalog number 15226
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RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection
Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs)
When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs
The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium
Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection)
Immunodensity pre-enrichment of untouched progenitor cells from cord blood